Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.

• Authors: HASAN Khomaini; FOŘTOVÁ Andrea; KOUDELÁKOVÁ Táňa; CHALOUPKOVÁ Radka; ISHITSUKA M.; NAGATA Yuji; DAMBORSKÝ Jiří; PROKOP Zbyněk
• Year of publication: 2011
• Type: Article in Periodical
• Magazine / Source: Applied and Environmental Microbiology
• MU Faculty or unit: Faculty of Science
• Doi: http://dx.doi.org/10.1128/AEM.02109-10
• Field: Biochemistry
• Keywords: Novel Haloalkane Dehalogenase DatA ;Agrobacterium tumefaciens C58
• Description: Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters.

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