Publication details

Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells

Investor logo
Investor logo
Investor logo
Authors

MAJEROVÁ Eva FOJTOVÁ Miloslava MOZGOVÁ Iva BITTOVÁ Miroslava FAJKUS Jiří

Year of publication 2011
Type Article in Periodical
Magazine / Source Plant Molecular Biology
MU Faculty or unit

Central European Institute of Technology

Citation
Doi http://dx.doi.org/10.1007/s11103-011-9816-7
Field Genetics and molecular biology
Keywords Nicotiana tabacum; Cell culture; Telomere; Hypomethylating agents; Zebularine; DNA methylation
Description Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info