Publication details
Výskyt a typizace izolátů Listeria monocytogenes v syrovém kravském mléce odebraném na farmách a z prodejních automatů
| Title in English | Occurrence and typing of Listeria monocytogenes isolated from raw cow´s milk collected on farms and from vending machines |
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| Authors | |
| Year of publication | 2012 |
| Type | Article in Periodical |
| Magazine / Source | Klinická mikrobiologie a infekční lékařství |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | listeria; bulk tank milk samples; ecological and conventional breeding; serotyping; macrorestriction analysis |
| Description | Background: Evaluation of the incidence and characteristics of L. monocytogenes in samples of raw cows milk collected on farms (bulk tank milk samples) and from vending machines. Material and methods: Detection of L. monocytogenes and enumeration was carried out according to EN/ISO 11290 – 1,2. Strains were characterised by serotyping and makrorestriction analysis using pulsed field gel electrophoresis. Results: The presence of L. monocytogenes was detected in 3,2% (11/346) of bulk tank milk samples and 1,8% (4/219) samples of raw cows milk from vending machines. Findings of L. monocytogenes in raw milk were sporadic, only on one farm strains of L. monocytogenes were detected repeatedly. Thirteen strains of L. monocytogenes belonged to serotype 1/2a, two strains to serotype 1/2b and one to serotype 4b. Macrorestriction analysis revealed considerable heterogeinity of profiles, nine different pulsotypes were detected. Pulsotype 711 was the most frequent. This pulsotype was found on three different farms. Conclusions: The incidence of L. monocytogenes in raw cows milk is relatively low in the Czech Republic. The results confirmed that some clones of L. monocytogenes from raw milk are identical with food and human strains. |