Tuberculosis (TB) is the most spread infection disease which is problematically curable. Difficulties related with unusual structure of cell wall (CW) of Mycobacterium tuberculosis, causative agents of TB, particularly with, for other organisms untypical composite, polysaccharides. Our effort to understand and describe structural details of key components of the mycobacterial CW have resulted in identification of several therapeutically famous glycosyltranferases (GTs) participated on synthesis of saccharide structures.
The knowledge of MT H37rv genome sequence enabled amplification of target genes and their cloning into the different expression vectors in order to their expression, purification and caracterization. Since the mycobacterial GTs are very problematic proteins (hydrophobic, often membrane or membrane associated), it was not possible prepare selected GTs in sufficient amount, purity and form suitable for the next characterization. This project is focused on wide testing of different systems and conditions in order to prepare proteins of required quality, quantity and for their structure-function characterization.

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