Publication details
USING DIFFERENT APPROACHES IN PREPARATION OF PUTATIVE MYCOBACTERIAL GLYCOSYLTRANSFERASES RV3782 AND RV0225 FOR CRYSTALLOGRAPHIC STUDIES
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | FEBS Journal Volume 275 Supplement 1 |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | tuberculosisů; mycobacterial glycosyltransferases; heterologic expression |
| Description | With using bioinformatics tools, several therapeutically famous glycosyltranferases (GTs) have been identified in Mycobacterium tuberculosis genom and genes rv3782 and rv0225 were amplified and cloned into the different expression vectors in order to provide their expression, purification and characterization. The task was complicated by the nature of glycosyltransferases, which are often unstable, hydrophobic and hard to isolate and purify. Therefore, the performance of various expression systems was explored in order to identify the best experimental routes. |
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