Project information
Development of a new typing system for the causative agent of syphilis, Treponema pallidum subsp. pallidum, focusing on proteomic differences
- Project Identification
- 17-31333A (kod CEP: NV17-31333A)
- Project Period
- 4/2017 - 12/2020
- Investor / Pogramme / Project type
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Ministry of Health of the CR
- Programme NV (2015 - 2022)
- MU Faculty or unit
- Faculty of Medicine
- Cooperating Organization
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General Teaching Hospital Praha
- Responsible person doc. MUDr. Ivana Kuklová, CSc.
- Responsible person MUDr. Hana Zákoucká
Treponema pallidum subsp. pallidum (TPA) is the causative agent of the sexually transmitted disease syphilis. Globally, over 10 million new cases of syphilis are diagnosed each year. With the advancement of genomics, two molecular typing systems have been developed to characterise clinical TPA strains; however, their typing efficiency and discriminatory power vary considerably. The aim of this project is to develop a new, highly reliable and effective molecular typing system, focusing on sequences encoding proteomic differences between TPA strains, which may underlie varying clinical manifestations. The discriminatory power of the typing system will be tested on genetically distinct clinical isolates and samples from various locations around the world. Knowledge of the genetic basis of current clinical TPA strains is also crucial in terms of ongoing efforts to develop a vaccine against syphilis. The application of the new typing system to clinical strains from the Czech Republic will provide information on the diversity and prevalence of individual TPA genotypes within our country. The molecular data will be correlated with available epidemiological data.
Publications
Total number of publications: 15
2021
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The Arg753Gln Polymorphism of Toll-Like Receptor 2 Has a Lower Occurrence in Patients with Syphilis, Suggesting Its Protective Effect in Czech and Slovak Individuals
INFECTION AND IMMUNITY, year: 2021, volume: 89, edition: 1, DOI
2020
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A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results
Plos one, year: 2020, volume: 15, edition: 8, DOI
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Genotyping of Treponema pallidumin Cuba (2018-2019): Increased Circulation of Recombinant Genotype and No New Treponema pallidum Subspeciesendemicum Infection Among Syphilis Patients
Sexually transmitted diseases, year: 2020, volume: 47, edition: 10, DOI
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No bejel among Surinamese, Antillean and Dutch syphilis diagnosed patients in Amsterdam between 2006-2018 evidenced by multi-locus sequence typing of Treponema pallidum isolates
PLoS ONE, year: 2020, volume: 15, edition: 3, DOI
2019
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A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis
PeerJ, year: 2019, volume: 6, edition: JAN 9 2019, DOI
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Directly Sequenced Genomes of Contemporary Strains of Syphilis Reveal Recombination-Driven Diversity in Genes Encoding Predicted Surface-Exposed Antigens
Frontiers in Microbiology, year: 2019, volume: 10, edition: JUL 31 2019, DOI
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Genetic Stability of the Typing Loci Used in the Multilocus Sequence Typing and Enhanced CDC Typing of Syphilis Treponemes
Sexually transmitted diseases, year: 2019, volume: 46, edition: 6, DOI
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MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants
Plos one, year: 2019, volume: 14, edition: 5, DOI
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Multilocus Sequence Typing of Treponema pallidum subsp. pallidum in Cuba From 2012 to 2017
The Journal of Infectious Diseases, year: 2019, volume: 219, edition: 7, DOI
2018
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Bejel in Cuba: molecular identification of Treponema pallidum subsp endemicum in patients diagnosed with venereal syphilis
Clinical Microbiology and Infection, year: 2018, volume: 24, edition: 11, DOI