Project information

Rational re-design of microbial enzymes involved in degradation of toxic organic pollutants.

Project Identification
ME 276
Project Period
6/1998 - 12/2002
Investor / Pogramme / Project type
Ministry of Education, Youth and Sports of the CR
MU Faculty or unit
Faculty of Science
Keywords
protein engineering, haloalkane dehalogenase, site-directed mutagenesis, biodegradation, detoxification, structure-function relationships, computer modelling

The main objective of the project is the investigation of structure-function relationships of the enzymes involved in degradation of toxic substances - halogenated aliphatic hydrocarbons. The structures of the proteins from the family of haloalkane dehalogenases will be rationaly modified using the methods of genetic engineering. The impact of the changes in proteins structure on activity and specificity will be tested using steady-state kinetic measurements. Design of the mutant proteins will be conducted using computer modelling. Mutant proteins will be constructed using site-directed mutagenesis techniques. General structure-function relationships will be formulated from derived experimental data.

Publications

Total number of publications: 18


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