Publication details

Identification of clinical isolates from the genus Staphylococcus by MALDI-TOF MS and conventional phenothypic methods

Investor logo
Investor logo


Year of publication 2012
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Staphylococci in general and S.aureus subsp. aureus in particular are very important human pathogens. More than 400 strains isolated from clinical specimens were identified by MALDI-TOF MS (Microflex; Bruker-Daltonics, Germany) and conventional phenotypic methods (API Staph; STAPHYtest 24; conventional tests). Type strains of 53 staphylococccal species and subspecies were also included in the study. MALDI-TOF MS successfully identified 85% of the clinical isolates to the species level (score >1.900), but not to the subspecies level. The isolates were assigned to 26 species. The MALDI-TOF MS and phenotypic results were concordant in more than 99%. Thirty-one clinical isolates were identified by MALDI-TOF MS as belonging to the complex S. intermedius/S. pseudintermedius. All but one strain of this complex were assigned to S. pseudintermedius by rep-PCR fingerprinting using the (GTG)5 primer. The MALDI-TOF mass spectra-based dendrogram constructed from the data on 53 type strains and data retrieved from the Bruker-Daltonics database revealed that the strains of the same species clustered together and certain branches showed a sufficiently good concordance with the Staphylococcus species groups defined by the phylogenetic analysis of 16S rRNA sequences. Our results indicate that MALDI-TOF MS is a very effective tool for the identification of staphylococci from clinical specimens that has the advantage of being simple, swift, and reliable and requiring low material cost.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info