Publication details

A combined immunoprecipitation and mass spectrometric approach to determine deltaNp63-interacting partners

Authors

HERNYCHOVÁ Lenka NEKULOVÁ Marta POTĚŠIL David MICHALOVÁ Eva ZDRÁHAL Zbyněk VOJTĚŠEK Bořivoj HOLČÁKOVÁ Jitka

Year of publication 2012
Type Article in Periodical
Magazine / Source Klinická onkologie
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.linkos.cz/files/klinicka-onkologie/174/4133.pdf
Field Oncology and hematology
Keywords antibodies; p63; immunoprecipitation; proteomics; mass spectrometry; protein; protein interactions
Description Expression of p63 is essential for the formation of epidermis and other stratifying epitheliA. Moreover p63 is highly expressed in several epithelial cancers and is involved in tumourige­nesis and controlling chemosensitivity. The identification of p63 interacting partners is essential for understanding the complex network of gene regulation managing epithelial development and could also help to reveal signalling pathways participating in UV damage response in human skin. We used a proteomic approach to identify proteins that interact with deltaNp63. Proteins were isolated by immunoprecipitation with deltaNp63 specific antibody and analysed by mass spectrometry. We identified 23 proteins as potential deltaNp63 binding partners that were not present in negative control samples. These results will be evaluated using other methods.
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