Publication details

Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels

Authors

KONEČNÁ Hana FRIDRICHOVÁ Danuše LOCHMANOVÁ Gabriela BÁRTA Jan ZDRÁHAL Zbyněk

Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Central European Institute of Technology

Citation
Doi http://dx.doi.org/10.1002/elps.201300084
Field Analytic chemistry
Keywords 2DE; Mixed detergent micelles; Tris-Tricine gel
Description Application of Tris-N-[Tris(hydroxymethyl)methyl]glycine gels for 2DE is hampered by formation of mixed CHAPS-SDS micelles resulting in typical swirling pattern in the low mass range, which makes reliable quantitative and qualitative gel evaluation impossible. Modification of 2DE strip equilibration procedure prevented the direct interaction between both detergents during equilibration process, thus substantially improving gel separation.
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