Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger

• Authors: DEMO Gabriel; FLIEDROVA Barbora; WEIGNEROVA Lenka; WIMMEROVÁ Michaela
• Year of publication: 2013
• Type: Conference abstract
• MU Faculty or unit: Central European Institute of Technology
• Description: We report the crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase overexpressed in Pichia pastoris. The best crystals were produced by further optimization using the hanging-drop vapour diffusion method. Diffraction data were collected at BESSY II Berlin (14.1 and 14.2). The data were processed by XDSAPP. The crystals belonged to space group P1. The beta-mannosidase in the native data set diffracted to 2.41 A resolution. Experimental phasing, model fitting and refinement are in progress.

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