Publication details

Novosphingobium barchaimii sp nov., isolated from hexachlorocyclohexane-contaminated soil

Authors

NIHARIKA Neha MOSKALÍKOVÁ Hana KAUR Jasvinder SEDLÁČKOVÁ Miroslava HAMPL Aleš DAMBORSKÝ Jiří PROKOP Zbyněk LAL Rup

Type Article in Periodical
Magazine / Source International Journal of Systematic and Evolutionary Microbiology
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1099/ijs.0.039826-0
Field Biochemistry
Keywords IDENTIFICATION; MICROORGANISMS; SPHINGOMONAS; SPHINGOBIUM; ACID
Description A yellow pigmented bacterial strain, designated LL02T was isolated from HCH contaminated soil from Spolana, Neratovice former Czech producer of Lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL02T occupied a distinct phylogenetic position in the Novophingobium cluster, showing the highest similarity with Novosphingobium resinovorum NCIMB 8767T (98.59%). The DNA-DNA relatedness values of strain LL02T with its phylogenetic neighbours were found to be less than 70% indicating that strain LL02T represents a novel species of the genus Novosphingobium. The DNA G+C content of strain LL02T was 67.72 plus minus 0 %. The major respiratory quinone present was ubiquinone Q10. The polar lipid profile of the strain LL02T also corresponded to those reported for other Novosphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sphingoglycolipids), thus supporting its identification as a member of the genus Novosphingobium.
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