Publication details

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2013-31 May 2013

Authors

AGOSTINI Cecilia ALBALADEJO R.G. APARICIO A. ARTHOFER Wolfgang BERREBI P. BOAG Peter T. CARBONE Ignazio CONROY Gabriel C. CORTESERO A.M. GONCALVES Evonnildo Costa COSTA Diogo COUTO Alvarina DE GIROLAMO Mirko DU Hao FU Shi-Jian GARRIDO-GARDUNO T. GETTOVÁ Lenka GILLES A. HAMOY Igor Guerreiro HERRERA C. M HEUSSLER Carina ISIDRO Eduardo JOSSO C. KRAPF Patrick LAMONT Robert W. LE RALEC A. LOPES Susana LUIS Carla LUO Hui MAHEO F. MARINO Ilaria A. M. MIEUZET L. MURRAY Brent W. OGBOURNE Steven M. PALLAVICINI Alberto PAREJO-FARNES C. PATARNELLO Tomaso PATY C. PEREIRA Carolina PINHO Catarina PINTO Pablo POINSOT D. POWELL Adrienne PUTMAN Alexander I. SANTORO Andre SANTOS Sidney SCHLICK-STEINER Birgit C. SCOTT Candace BARBOSA Maria Silvanira VETEŠNÍKOVÁ ŠIMKOVÁ Andrea SIMON J. -C. SOLE-CAVA Antonio STEINER Florian M. SUN Zhengxin TORBOLI Valentina TREDWAY Lane P. DE GROOT Peter J. Van Coeverden VASCONCELLOS Anderson VAZQUEZ-DOMINGUEZ E. WANG Deng-Qiang WANG Yu-Xiang WEI Qi-Wei ZANE Lorenzo ZHANG Shu-Huan

Year of publication 2013
Type Article in Periodical
Magazine / Source Molecular Ecology Resources
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1111/1755-0998.12140
Field Genetics and molecular biology
Keywords microsatellites 454 GS-FLX Titanium sequencing cyprinids Barbus cross-amplification hybrid zones
Description Using cost-effective 454 GS-FLX Titanium sequencing technology, microsatellite markers were isolated de novo for Barbus meridionalis. Five multiplex sets including 28 newly and two previously-developed loci were optimized using allopatric populations of both B. barbus and B. meridionalis. Our study revealed the high success rate of cross-species amplification in two Balkan species, B. cyclolepis and B. balcanicus.
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