Publication details

Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

Authors

BALINTOVÁ Jana PLUCNARA Medard VIDLÁKOVÁ Pavlína POHL Radek HAVRAN Luděk FOJTA Miroslav HOCEK Michal

Year of publication 2013
Type Article in Periodical
Magazine / Source Chemistry - A European Journal
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04
Doi http://dx.doi.org/10.1002/chem.201301868
Field Biochemistry
Keywords DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry
Description Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
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