Publication details

Production of human proteins using well-established and alternative recombinant expression hosts

Authors

MRÁZKOVÁ Jana JANČAŘÍKOVÁ Gita POKORNÁ Marie KYSEĽ Peter WIMMEROVÁ Michaela

Year of publication 2014
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description In our work, we demonstrate using several approaches of production of a difficult-obtainable human lectin. We tried production of this protein using well-established E. coli expression host but this way failed because the protein is produced in the insoluble form only. Hence refolding, in vitro denaturation/renaturation and some solubility-enhancing tags were utilized to obtain protein in soluble form. Finally, it turned out that the L. tarentolae possibilities of the disulfide bond formation and the human-like glycosylation are crucial for a sufficient production of our target protein and it seems to be the very effective system for a routine production of human proteins in general.
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