Publication details

Detection of Minimal Residual Disease in Mantle Cell Lymphoma. Establishment of Novel Eight-Color Flow Cytometry Approach

Authors

CHOVANCOVÁ Jana BERNARD Tomáš STEHLÍKOVÁ Olga ŠÁLEK David JANÍKOVÁ Andrea MAYER Jiří DOUBEK Michael

Year of publication 2015
Type Article in Periodical
Magazine / Source Cytometry Part B: Clinical Cytometry
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/cyto.b.21210/epdf
Doi http://dx.doi.org/10.1002/cytob.21210
Field Oncology and hematology
Keywords mantle cell lymphoma;minimal residual disease;flow cytometry
Attached files
Description Backround: Minimal residual disease (MRD) detection is an essential tool for therapy response assessment in a considerable number of hematooncologic disorders including mantle cell lymphoma (MCL). Flow cytometry (FCM) ranks among the most effective approaches which allow rapid sample processing and compete successfully with highly sensitive molecular methods like polymerase chain reaction (PCR). Since FCM is ordinarily applied to detect MRD in B-lineage diseases like chronic lymphocytic leukemia (CLL), a similar method could be used in MCL. We decided to test our novel 8-color FCM approach in MCL MRD detection. Methods: Employing an 8-color FCM protocol designed by us, the expression of 24 selected surface antigens in a cohort of 30 patients with newly diagnosed leukemic MCL and 20 normal controls were compared to one another to establish markers that can reliably distinguish normal B lymphocytes from the MCL population. The sensitivity of the designed protocol was tested using serial dilution studies. Results: Although MCL presents high immunophenotypic variability the combination of CD20/23/5/19/200/62L/3/45 appears to be very favorable in flow cytometric MRD measurement. Conclusions: Our 8-color FCM protocol could easily detect MRD in MCL patients and reaches a sensitivity of up to 2×10-4. This article is protected by copyright. All rights reserved.
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