Publication details

On Proper Simulation of Chromatin Structure in Static Images As Well As in Time-Lapse Sequences in Fluorescence Microscopy

Authors

SVOBODA David ULMAN Vladimír PETERLÍK Igor

Type Article in Proceedings
Conference Proceedings of 2015 IEEE International Symposium on Biomedical Imaging
MU Faculty or unit

Faculty of Informatics

Citation
Doi http://dx.doi.org/10.1109/ISBI.2015.7163972
Field Informatics
Keywords Simulation; Synthetic cell; Chromatin structure; Nucleus deformation; Linear elasticity; FEM
Description In fluorescence microscopy, where the benchmark datasets for validating the various image analysis methods are difficult to obtain, a great demand is either for manually annotated real image data or for computer generated ones. In the last two decades, the latter case has become more and more accessible due to an increasing computer capabilities. However, the development of elaborate models, especially in the field of fluorescence microscopy imaging, is less progressive. In this paper, we propose a novel approach, based on well established concepts, to properly imitate the structure of chromatin inside the cell nucleus as well as its dynamics. The performance of the approach was quantitatively evaluated against the real data. The results show that the produced images are sufficiently plausible and visually resemble their real counter parts, both for fixed and living cells.
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