Publication details

MiR-21, miR-34a, miR-198 and miR-217 as diagnostic and prognostic biomarkers for chronic pancreatitis and pancreatic ductal adenocarcinoma.

Authors

VYCHYTILOVÁ Petra KISS Igor KLUSOVÁ Soňa HLAVSA Jan PROCHÁZKA Vladimír KALA Zdeněk MAZANEC Jan HAUSNEROVÁ Jitka KŘEN Leoš HERMANOVÁ Markéta LENZ Jiří KARÁSEK Petr VYZULA Rostislav SLABÝ Ondřej

Year of publication 2015
Type Article in Periodical
Magazine / Source Diagnostic Pathology
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.diagnosticpathology.org/content/pdf/s13000-015-0272-6.pdf
Doi http://dx.doi.org/10.1186/s13000-015-0272-6
Field Oncology and hematology
Keywords microRNA; pancreatic ductal adenocarcinoma; diagnostic biomarkers; prognostic biomarkers; chronic pancreatitis
Attached files
Description Background: Pancreatic ductal adenocarcinoma is an aggressive malignancy with late presentation, metastatic potential and very poor prognosis. Therefore, there is an urgent need for novel diagnostic and prognostic biomarkers. MicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression. Altered expression of microRNAs has been reported in wide range of malignancies, including pancreatic ductal adenocarcinoma. The aim of this study was to analyze the expression of selected microRNAs in normal pancreas, chronic pancreatitis and pancreatic ductal adenocarcinoma tissues and evaluate their diagnostic and prognostic potential. Findings: Using quantitative real-time PCR, expression levels of 4 microRNAs were examined in 74 tumor tissues, 18 tissues of chronic pancreatitis and 9 adjacent normal tissues and correlated with clinicopathological features of patients. Expression levels of miR-21, miR-34a and miR-198 were significantly higher, whereas levels of miR-217 were significantly lower in pancreatic ductal adenocarcinomas compared to healthy tissues and tissues of chronic pancreatitis. Moreover, increased expression of miR-21 and miR-198 was significantly associated with shorter disease free survival and overall survival. Conclusions: Our data suggest that altered expression of examined microRNAs is related to neoplastic transformation and progression of the disease and these microRNAs could serve as diagnostic and prognostic biomarkers for pancreatic ductal adenocarcinoma.
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