Publication details

Gelatin templated gold nanostructured electrodes for sensitive glucose detection

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Authors

JUŘÍK Tomáš FARKA Zdeněk KOVÁŘ David PODEŠVA Pavel FORET František SKLÁDAL Petr

Year of publication 2015
Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Citation
Description Due to the importance of glucose in physiological processes, sensitive, rapid and reliable methods for its detection are required. The biocatalytic sensors provide low limits of detection but the enzymatic layer is volatile. Sensors that are not based on enzymes are very stable in all conditions, however worse detection limits are typically obtained. One of the ways to achieve an improved sensitivity of the detection is by developing of a sensor with optimized surface morphology. In this work gelatin templated gold nanostructures were fabricated in order to improve the sensitivity of glucose detection. The 3D cavities were made by crosslinking of gelatine layer on the gold surface and nanostructured surface was formed in these cavities by electrodeposition of gold from the electroplating solution. Gel layers on the electrodes were cleaned by KOH and chromic acid. Scanning electron microscopy and atomic force microscopy were used for characterisation of the created surfaces. The glucose was detected by a direct electrochemical oxidation during cyclic voltammetry in alcaline solution. Limit of detection of 10 µM was achieved in water samples. For the detection of glucose in serum, the samples were first deproteinized in acetone, centrifugated and the pH was adjusted by KOH. The sensor was able to detect 1.3 mM glucose in diluted deproteinised human serum samples with negligible effect of interferents present in human blood serum. The results were verified using commercial glucometer ACCU-CHEK Active® and the standard kit for photometrical detection of glucose BioLaTest GLU L 500 S.
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