Publication details
NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | Capillary electrophoresis |
| Description | Capillary electrophoresis (CE) with double opposite end injection (DOEI) and contactless conductivity detection (C4D) was used in a novel approach for diagnosis of cystic fibrosis (CF). A simple, fast and inexpensive "skin wipe" technique for sweat sampling was developed as a time-saving substitute for conventional, iontophoretic sweat sampling. Saliva sample was also obtained. A number of major target analytes (inorganic/organic anions and inorganic cations) were quantified simultaneously in these samples. By applying principal component analysis (PCA) to ion concentration ratios, rather than to individual ion concentrations, more accurate diagnostic tool for CF was obtained. We demonstrate that by using the developed approach and comparing a group of healthy individuals to patients diagnosed with CF, two clearly distinguished clusters can be observed without any data overlap. This approach may thus set a basis for a new method to diagnose CF more reliably. |