Publication details

Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material

Authors

ŠVEC Pavel DE BEL Annelies SEDLÁČEK Ivo PETRÁŠ Petr GELBÍČOVÁ Tereza ČERNOHLÁVKOVÁ Jitka MAŠLAŇOVÁ Ivana VARBANOVOVÁ Ivana CNOCKAERT Margo ECHAHIDI Fedoua VANDAMME Peter PANTŮČEK Roman

Year of publication 2015
Type Article in Periodical
Magazine / Source International Journal of Systematic and Evolutionary Microbiology
MU Faculty or unit

Faculty of Science

Citation
Web http://ijs.sgmjournals.org/content/journal/ijsem/10.1099/ijs.0.000220
Doi http://dx.doi.org/10.1099/ijs.0.000220
Field Microbiology, virology
Keywords coagulase-negative staphylococci; Staphylococcus petrasii subsp. pragensis; rep-PCR; molecular phylogeny; MALDI-TOF mass spectrometry; fatty acid methyl ester analysis; DNA-DNA hybridization; automated ribotyping
Attached files
Description Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA–DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356T (=CCM 8529T=LMG 28327T).
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