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Biophysical and electrochemical studies of protein-nucleic acid interactions

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BOWATER Richard P. COBB Andrew M. PIVOŇKOVÁ Hana HAVRAN Luděk FOJTA Miroslav

Year of publication 2015
Type Article in Periodical
Magazine / Source Monatshefte fur Chemie
MU Faculty or unit

Central European Institute of Technology

Field Biophysics
Keywords Bioorganic chemistry; Biosensors; Electrochemistry; Electron transfer; Electrophoresis; Nucleic acids; Proteins; Voltammetry
Description This review is devoted to biophysical and electrochemical methods used for studying protein-nucleic acid (NA) interactions. The importance of NA structure and protein-NA recognition for essential cellular processes, such as replication or transcription, is discussed to provide background for description of a range of biophysical chemistry methods that are applied to study a wide scope of protein-DNA and protein-RNA complexes. These techniques employ different detection principles with specific advantages and limitations and are often combined as mutually complementary approaches to provide a complete description of the interactions. Electrochemical methods have proven to be of great utility in such studies because they provide sensitive measurements and can be combined with other approaches that facilitate the protein-NA interactions. Recent applications of electrochemical methods in studies of protein-NA interactions are discussed in detail. [GRAPHICS] .
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