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A new panel of proteins associated with metastasis in low-grade breast cancer: a role in cell migration and invasiveness

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Year of publication 2016
Type Conference abstract
Description Introduction: We recently identified a new panel of proteins associated with lymph-node metastasis of low-grade luminal A breast cancer using proteomics, transcriptomics and immunohistochemistry on the set of well characterized 96 tumors [Bouchal et al., Combined proteomics and transcriptomics identifies carboxypeptidase B1 and nuclear factor ?B (NF-?B) associated proteins as putative biomarkers of metastasis in low grade breast cancer, Mol. Cell. Proteomics, 14(7), 1814-1830 (2015)]. Our subsequent research has focused on the role of these proteins in migration and invasiveness of cancer cells and on understanding their molecular role in these processes. Methods: The role of CPB1, PDLIM2, RNF25 and TRAF3IP2 proteins in migration and invasiveness of transiently transfected MCF7 breast cancer cells was studied using xCELLigence system (Roche) and Transwell assay (Corning). In parallel, SWATH proteomics analyses of corresponding whole proteome lysates of MCF7 cells collected 24, 48 and 72 h after the transfection were performed, complemented by pull-down-MS assays on protein-protein interactions. Results: Analyses of xCELLigence and Transwell data have confirmed statistically significant connection of increased levels of CPB1, PDLIM2, RNF25 and TRAF3IP2 with MCF7 cell migration and/or invasiveness. This work proceeds by studying migratory and invasive properties in 3D cultures. Analysis of proteome profiles and interaction network obtained by next-generation SWATH proteomics has revealed new potentially functionally interacting proteins associated with cell proliferation and adhesion. Acknowledgement: The work was supported by Czech Science Foundation (project No. 14-19250S), by the project MEYS - NPS I - LO1413 and by MH CZ - DRO (MMCI, 00209805). Conference participation was supported by the grant from Masaryk University (MUNI/A/1265/2015).
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