Tri- and tetravalent mannoclusters cross-link and aggregate BC2L-A lectin from Burkholderia cenocepacia

• Authors: CSÁVÁS Magdolna; MALINOVSKÁ Lenka; PERRET Florent; GYURKÓ Milán; ILLYÉS Zita Tünde; WIMMEROVÁ Michaela; BORBÁS Anikó
• Year of publication: 2017
• Type: Article in Periodical
• Magazine / Source: Carbohydrate Research
• MU Faculty or unit: Central European Institute of Technology
• Web: https://www.sciencedirect.com/science/article/pii/S0008621516303160?via%3Dihub
• Doi: http://dx.doi.org/10.1016/j.carres.2016.11.008
• Field: Biochemistry
• Keywords: Burkholderia cenocepacia; Mannose-binding lectin; Glycoclusters; Click reaction; Anti-adhesion therapy
• Description: The opportunistic Gram-negative bacterium Burkholderia cenocepacia causes lethal infections in cystic fibrosis patients. Multivalent mannoside derivatives were prepared as potential inhibitors of lectin BC2L-A, one of the virulence factors deployed by B. cenocepacia in the infection process. The interaction between the glycoclusters and the mannose binding BC2L-A lectin from B. cenocepacia was examined by isothermal microcalorimetry, surface plasmon resonance, inhibition of yeast agglutination and analytical ultracentrifugation.

Related projects

• Glykoklastry kalix[4]aren/C-oligosacharidy pro studium selektivity interakcí s lektiny
• Czech Infrastructure for Integrative Structural Biology