Publication details

Wild-type p53 binds to MYC promoter G-quadruplex

Authors

PETR Marek HELMA Robert POLÁŠKOVÁ Alena KREJČÍ Aneta DVOŘÁKOVÁ Zuzana KEJNOVSKÁ Iva NAVRÁTILOVÁ Lucie ADÁMIK Matej VORLÍČKOVÁ Michaela BRÁZDOVÁ Marie

Year of publication 2016
Type Article in Periodical
Magazine / Source Bioscience Reports
Citation
Web http://dx.doi.org/10.1042/BSR20160232
Doi http://dx.doi.org/10.1042/BSR20160232
Keywords DNA-protein interaction; G-quadruplex; MYC; p53 protein
Description G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumour suppressor p53 plays a critical role in regulatory pathways leading to cell cycle arrest, DNA repair and apoptosis. In addition to transcriptional regulation mediated via sequence-specific DNA binding, p53 can selectively bind various non-B DNA structures. In the present study, wild-type p53 (wtp53) binding to G-quadruplex formed by MYC promoter nuclease hypersensitive element (NHE) III1 region was investigated. Wtp53 binding to MYC G-quadruplex is comparable to interaction with specific p53 consensus sequence (p53CON). Apart from the full-length wtp53, its isolated C-terminal region (aa 320-393) as well, is capable of high-affinity MYC G-quadruplex binding, suggesting its critical role in this type of interaction. Moreover, wtp53 binds to MYC promoter region containing putative G-quadruplex motif in two wtp53-expressing cell lines. The results suggest that wtp53 binding to G-quadruplexes can take part in transcriptional regulation of its target genes.