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Wild-type p53 binds to MYC promoter G-quadruplex

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PETR Marek HELMA Robert POLÁŠKOVÁ Alena KREJČÍ Aneta DVOŘÁKOVÁ Zuzana KEJNOVSKÁ Iva NAVRÁTILOVÁ Lucie ADÁMIK Matej VORLÍČKOVÁ Michaela BRÁZDOVÁ Marie

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Bioscience Reports
Citace
www http://dx.doi.org/10.1042/BSR20160232
Doi http://dx.doi.org/10.1042/BSR20160232
Klíčová slova DNA-protein interaction; G-quadruplex; MYC; p53 protein
Popis G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumour suppressor p53 plays a critical role in regulatory pathways leading to cell cycle arrest, DNA repair and apoptosis. In addition to transcriptional regulation mediated via sequence-specific DNA binding, p53 can selectively bind various non-B DNA structures. In the present study, wild-type p53 (wtp53) binding to G-quadruplex formed by MYC promoter nuclease hypersensitive element (NHE) III1 region was investigated. Wtp53 binding to MYC G-quadruplex is comparable to interaction with specific p53 consensus sequence (p53CON). Apart from the full-length wtp53, its isolated C-terminal region (aa 320-393) as well, is capable of high-affinity MYC G-quadruplex binding, suggesting its critical role in this type of interaction. Moreover, wtp53 binds to MYC promoter region containing putative G-quadruplex motif in two wtp53-expressing cell lines. The results suggest that wtp53 binding to G-quadruplexes can take part in transcriptional regulation of its target genes.