Publication details

Molecular survey of arthropod-borne pathogens in sheep keds (Melophagus ovinus), Central Europe.

Authors

RUDOLF Ivo BETÁŠOVÁ Lenka BISCHOF Vlastimil VENCLÍKOVÁ Kristýna BLAŽEJOVÁ Hana MENDEL Jan HUBÁLEK Zdeněk KOSOY Michael

Year of publication 2016
Type Article in Periodical
Magazine / Source Parasitology Research
MU Faculty or unit

Faculty of Science

Citation
Web https://link.springer.com/content/pdf/10.1007%2Fs00436-016-5175-2.pdf
Doi http://dx.doi.org/10.1007/s00436-016-5175-2
Field Microbiology, virology
Keywords Melophagus ovinus; Bartonella melophagi; Rickettsiae; Arboviruses; Borrelia burgdorferi; Babesia spp
Description In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phagocytophilum, “Candidatus Neoehrlichia mikurensis,” and Babesia spp. All ked pools were positive for Bartonella DNA. The sequencing of the amplified fragments of the gltA and 16S-23S rRNA demonstrated a 100 % homology with Bartonella melophagi previously isolated from a sheep ked and from human blood in the USA. The identification of B. melophagi in sheep keds in Central Europe highlights needs extending a list of hematophagous arthropods beyond ticks and mosquitoes for a search of emerging arthropod-borne pathogens. © 2016, Springer-Verlag Berlin Heidelberg.
Related projects: