Publication details

Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species

Authors

SYCHROVÁ Eliška PRIEBOJOVÁ Jana SMUTNÁ Marie NOVÁKOVÁ Kateřina KOHOUTEK Jiří HILSCHEROVÁ Klára

Year of publication 2016
Type Article in Periodical
Magazine / Source Harmful Algae
MU Faculty or unit

Faculty of Science

Citation
Web http://www.sciencedirect.com/science/article/pii/S156898831630021X
Doi http://dx.doi.org/10.1016/j.hal.2016.11.002
Field Hydrology and limnology
Keywords Retinoic acid receptor; Cyanobacteria; Biomass extracts; Exudates; All-trans-retinoic acid; 9-Cis retinoic acid; Retinoid-like activity; Microcystin; Light conditions
Description Phytoplankton can produce various bioactive metabolites, which may affect other organisms in the aquatic environment. This study provides the first information on the total retinoid-like activity associated with both intracellular and extracellular metabolites produced by selected phytoplankton species that could play a role in teratogenic effects and developmental disruption in exposed organisms. The studied species included a coccoid cyanobacteria, a filamentous cyanobacteria and a green alga, all of which commonly occur in freshwater bodies in Europe. Methanolic extracts from cellular material and extracellular exudates were prepared from cultures cultivated in two light-intensity variants with five replicates for each species. The retinoid-like activity was evaluated by in vitro assays along with chemical analyses of two potent retinoic acids (all-trans retinoic acid (ATRA) and 9cis-RA). The mean total retinoid-like activity of metabolites produced by the three studied species representing different phytoplankton taxonomic groups ranged from 705 to 5572 ng ATRA equivalent/g dry matter corresponding to 0.064-0.234 ng ATRA/10(6) cells. Retinoid-like activity was found in the cellular extracts of all species, while only the extracellular exudates of cyanobacteria exhibited detectable activity (41-1081 ng ATRA/L). The greatest extracellular as well as total (extra- and intra- cellular together) retinoid-like activity was detected for Microcystis aeruginosa. The two potent retinoic acids studied were more frequently detected in cellular extracts than in extracellular exudates of all species. The results indicate possible influence of light intensity and cell density on the production of metabolites with retinoid-like activity and the cyanotoxin microcystin by the studied species.
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