Publication details

Metabolic stress regulates ERK activity by controlling KSR-RAF heterodimerization

Authors

VERLANDE Amandine KRAFČÍKOVÁ Michaela POTĚŠIL David TRANTÍREK Lukáš ZDRÁHAL Zbyněk ELKALAF M. TRNKA J. SOUČEK Karel RAUCH N. RAUCH J. KOLCH W. ULDRIJAN Stjepan

Type Article in Periodical
Magazine / Source EMBO reports
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.15252/embr.201744524
Keywords cell cycle arrest; cell survival; melanoma; metabolic stress; RAF-ERK signaling
Description Altered cell metabolism is a hallmark of cancer, and targeting specific metabolic nodes is considered an attractive strategy for cancer therapy. In this study, we evaluate the effects of metabolic stressors on the deregulated ERK pathway in melanoma cells bearing activating mutations of the NRAS or BRAF oncogenes. We report that metabolic stressors promote the dimerization of KSR proteins with CRAF in NRAS-mutant cells, and with oncogenic BRAF in BRAF(V600E)-mutant cells, thereby enhancing ERK pathway activation. Despite this similarity, the two genomic subtypes react differently when a higher level of metabolic stress is induced. In NRAS-mutant cells, the ERK pathway is even more stimulated, while it is strongly downregulated in BRAF(V600E)-mutant cells. We demonstrate that this is caused by the dissociation of mutant BRAF from KSR and is mediated by activated AMPK. Both types of ERK regulation nevertheless lead to cell cycle arrest. Besides studying the effects of the metabolic stressors on ERK pathway activity, we also present data suggesting that for efficient therapies of both genomic melanoma subtypes, specific metabolic targeting is necessary.
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