Publication details

Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping

Authors

KOSTOLANSKÁ Katarína PEŠ Ondřej ZENDULKA Ondřej MÁCHAL Jan JUŘICA Jan

Year of publication 2019
Type Article in Periodical
Magazine / Source Chemical Papers
MU Faculty or unit

Faculty of Medicine

Citation
Web https://link.springer.com/article/10.1007/s11696-019-00682-4#citeas
Doi http://dx.doi.org/10.1007/s11696-019-00682-4
Keywords Cytochrome P450; Phenotype; Lansoprazole; 5-Hydroxylansoprazole; Lansoprazole sulfone; Liquid chromatography; Mass spectrometry; Metabolites;
Description A simple and sensitive liquid chromatography method with absorbance or mass spectrometry detection was developed and validated for the determination of lansoprazole, lansoprazole sulfone, and 5-hydroxylansorpazole in human serum with omeprazole as an internal standard. Analytes were, after an extraction step with a mixture of isopropyl alcohol: dichloromethane (2:8, v/v), separated in a gradient elution (acetonitrile: 20 mM ammonium formate) and detected at 280 and 300 nm or by monitoring selective reactions in a triple quadrupole mass analyzer. The limits of detection were, for all analytes, below 2 ng/mL and 3 pg/mL in the LC UV and LC MS method, respectively. The combined linear dynamic range exceeded 5 orders of magnitude. The method was successfully applied in the assessment of CYP2C19 and CYP3A4 metabolic phenotypes in ST-elevated myocardial infarction patients receiving novel anticoagulant treatment.
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