Publication details
Detection of antibodies to Borrelia burgdorferi s.l. in wild small mammals and sensitivity of PCR and cultivation
| Authors | |
|---|---|
| Year of publication | 2019 |
| Type | Article in Periodical |
| Magazine / Source | Veterinary Microbiology |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.sciencedirect.com/science/article/pii/S037811351830748X?via%3Dihub |
| Doi | http://dx.doi.org/10.1016/j.vetmic.2019.02.004 |
| Keywords | Lyme borreliosis rodents seroprevalence spirochetes ticks vector-borne diseases |
| Description | The aim of of this study was to determine the seroprevalence of antibodies to Borrelia burgdorferi s.l. in wild small mammals and to detect Borrelia burgdorferi s.l. directly by cultivation and PCR. Wild small mammals (n = 691) were trapped in years 2010 – 2014 in three localities of the Czech Republic. Heart rinses (n = 340) and sera (n = 351) were examined by modified indirect ELISA. Antibodies to Borrelia burgdorfei s.l. were detected in 10 % (47/453) of Apodemus flavicollis, 6 % (2/31) of Apodemus sylvaticus, 9 % (3/32) of Apodemus agrarius, 20 % (30/151) of Clethrionomys glareolus and 12 % (2/17) of Sorex araneus; Microtus arvalis (n = 6) and Talpa europea (n = 1) were negative. Mean positivity rate for five years of collection was 12 % with mean annual positivity rate 10 %, 17 %, 20 %, 2 % and 7 % in individual years. |