Publication details

Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex

Authors

KOTÁSKOVÁ Iva OBRUČOVÁ Hana LÝČKOVÁ Veronika RŮŽIČKA Filip FREIBERGER Tomáš

Type Article in Periodical
Magazine / Source Journal of Clinical Microbiology
MU Faculty or unit

Faculty of Medicine

Citation
Web http://dx.doi.org/10.1128/JCM.00135-19
Doi http://dx.doi.org/10.1128/JCM.00135-19
Keywords Candida; Candida parapsilosis complex; MALDI-TOF; fluorescent capillary electrophoresis; fungi
Description Candida parapsilosis complex (psilosis complex) has three genetically distinct but not easily phenotypically distinguishable cryptic species, C. parapsilosis sensu stricto, Candida metapsilosis, and C. orthopsilosis. It has been reported as one of the most important non-albicans agents commonly detected in invasive candidiasis (1–3). Each psilosis complex species manifests a unique epidemiology, virulence, and antifungal susceptibility (4); thus, precise identification can be of clinical interest, and various molecular methods are being developed (5–7). Our previous studies (5, 8) reported the capability of ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) to distinguish between various Candida species, including psilosis complex, by a small but constant 0.4-bp difference between C. parapsilosis and Candida orthopsilosis. The actual positions of the size standard fragments are plotted against the expected size of each size standard fragment, which defines the function of these variables. The size (bp) of an analyzed fragment is then calculated according to this function; therefore, decimal values are being reported. The 0.4-bp size difference is presumably caused by the different mobilities of singlestranded DNA (ssDNA) fragments, resulting from their different primary structures.
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