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Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex

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KOTÁSKOVÁ Iva OBRUČOVÁ Hana LÝČKOVÁ Veronika RŮŽIČKA Filip FREIBERGER Tomáš

Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Clinical Microbiology
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1128/JCM.00135-19
Klíčová slova Candida; Candida parapsilosis complex; MALDI-TOF; fluorescent capillary electrophoresis; fungi
Popis Candida parapsilosis complex (psilosis complex) has three genetically distinct but not easily phenotypically distinguishable cryptic species, C. parapsilosis sensu stricto, Candida metapsilosis, and C. orthopsilosis. It has been reported as one of the most important non-albicans agents commonly detected in invasive candidiasis (1–3). Each psilosis complex species manifests a unique epidemiology, virulence, and antifungal susceptibility (4); thus, precise identification can be of clinical interest, and various molecular methods are being developed (5–7). Our previous studies (5, 8) reported the capability of ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) to distinguish between various Candida species, including psilosis complex, by a small but constant 0.4-bp difference between C. parapsilosis and Candida orthopsilosis. The actual positions of the size standard fragments are plotted against the expected size of each size standard fragment, which defines the function of these variables. The size (bp) of an analyzed fragment is then calculated according to this function; therefore, decimal values are being reported. The 0.4-bp size difference is presumably caused by the different mobilities of singlestranded DNA (ssDNA) fragments, resulting from their different primary structures.
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