Publication details

Biochemical and cytological changes of tobacco BY-2 cells under diclofenac impact

Authors

SVOBODNÍKOVÁ Lucie KUMMEROVÁ Marie ZEZULKA Štěpán BABULA Petr

Year of publication 2019
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Studies focusing on environmental contamination have shown that besides “classic” persistent pollutants, pseudo-persistent emerging contaminants are also present. As highly specific biologically active substances, pharmaceuticals and pharmaceutically active substances entering the environment can influence non-target organisms including plants. One of the commonly detected and monitored pharmaceuticals in water is a non-steroidal anti-inflammatory drug diclofenac (DCF). Plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity of wide range of inorganic and organic pollutants at the cellular and subcellular levels. The question is, if suspension BY-2 can be used even for drug toxicity evaluation. Results of this study suggest that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, mitotic index, suspension density and dry weight. DCF-induced oxidative stress documented by increased production of total reactive oxygen species (ROS) triggered apoptotic alterations in BY-2 cells leading to programmed cell death – occurrence of acidic compartments and autophagic vacuoles in BY-2 cells and apoptotic chromatin condensation and DNA fragmentation in their nuclei. Due to its prompt and sensitive response tobacco BY-2 cell suspension is suitable for evaluation of phytotoxicity of drugs.
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