Mikrobiom jícnu, žaludku a duodena u pacientů s gastroezofageální refluxní chorobou - pilotní studie
|Title in English
|Oesophageal, stomach and duodenum microbioma in patients with gastroesophageal reflux disease - a pilot study
|Year of publication
|MU Faculty or unit
|The host microbiome may be an important factor in the etiopathogenesis of gastroesophageal reflux disease (GERD), which significantly increases the risk of developing esophagitis (RE), Barrett's esophagus (BE), and esophageal adenocarcinoma (EAC). The aim of the pilot metagenomic study was to characterize bacterial biota in samples from the esophagus (normal and possibly pathological tissue), stomach (body and antrum) and duodenum from GERD patients and from healthy controls. From a total of 74 biopsy specimens obtained in endoscopic examination of 2 patients with RE, 4 with BE, 4 with EAC and 6 healthy controls, nucleic acid was isolated using the All Prep DNA / RNA / miRNA Universal Kit (Qiagen). The 16S rDNA PCR products from the V4 region were sequenced on the Illumina MiniSeq platform. A total of 20 strains, 86 orders, 184 families and 295 bacterial genera were identified in the samples. The most numerous strains in all samples were Proteobacteria 45%, Firmicutes 39%, Bacteroidetes 9%, Actinobacteria 3% and Fusobacteria 1%. Streptococcus sp. and Prevotella sp. with a lower proportion of Veillonella sp. The diversity of local community species (alpha diversity) did not significantly differ between samples from patients with individual pathologies / healthy controls (Shannon Diversity Index: 2.48 RE; 2.51 BE; 2.32 EAC and 2.52 healthy controls), however a tendency of reduced diversity in samples from EAC patients was observed. In the esophagus samples from patients with RE and BE, there was a trend of increasing gram-negative taxa (Fusobacterium, Campylobacter), while an increase in the Campylobacter and Lactobacillus taxa (non-metric multidimensional scaling) was observed in the EAC patient group. Following the pilot data, we will analyze the microbiome of the upper gastrointestinal tract under physiological and pathological conditions in a larger number of patients with GERD. Knowledge of microbiome and its possible diversity in these localities can contribute to earlier diagnosis and optimization of the algorithm of secondary preventive measures in the development and treatment of BE and EAC in patients with GERD.