Publication details
UTILIZATION OF MICROPREPARATIVE FAST FOCUSING BY A NEW WIDE pH RANGE ELECTROLYTE SYSTEM BASED ON BIDIRECTIONAL ISOTACHOFORESIS
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | CHEMICKÉ LISTY |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/3394/3350 |
| Keywords | electrolyte system |
| Description | We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique. |