Publication details

Voltammetric behavior of a candidate anticancer drug roscovitine at carbon electrodes in aqueous buffers and a cell culture medium

Authors

ORSAG P. HAVRAN L. FOJT L. COUFAL J. BRAZDA V. FOJTA Miroslav

Year of publication 2019
Type Article in Periodical
Magazine / Source Monatshefte fur Chemie
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://link.springer.com/article/10.1007%2Fs00706-018-2333-5
Doi http://dx.doi.org/10.1007/s00706-018-2333-5
Keywords Roscovitine; Anticancer drug; Carbon electrodes; Voltammetry; Cell culture medium
Description Roscovitine is a candidate anticancer drug acting as an inhibitor of cyclin-dependent kinases which are involved in cell-cycle control and in apoptosis triggering. In our present study,we show for the first time that roscovitine yields a well-developed, analytically useful signal due to its irreversible electrochemical oxidation in alkaline range of pH (while in acidic media, its oxidation process is apparently rather complex and corresponding voltammetric signals are less suitable for analytical purposes). We demonstrate the possibility of voltammetric determination of the drug in buffer solutions (down to submicromolar concentrations of roscovitine) as well as in cell culture medium (down to micromolar concentrations) using in-house-fabricated pyrolytic graphite or screen-printed carbon electrodes. [GRAPHICS] .
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