Publication details

Associations between the presence of specific antibodies to the West Nile Virus infection and candidate genes in Romanian horses from the Danube delta

Authors

STEJSKALOVA K. JANOVA E. HORECKY C. HORECKA E. VACLAVEK P. HUBALEK Z. RELLING K. CVANOVÁ Michaela D'AMICO G. MIHALCA A. D. MODRY D. KNOLL A. HORIN P.

Year of publication 2019
Type Article in Periodical
Magazine / Source MOLECULAR BIOLOGY REPORTS
MU Faculty or unit

Faculty of Medicine

Citation
Web http://dx.doi.org/10.1007/s11033-019-04900-w
Doi http://dx.doi.org/10.1007/s11033-019-04900-w
Keywords West Nile virus; Horse; Restriction fragment length polymorphism; Microsatellite; SLC11A1; TLR4; MHC; NKR; Polymorphism
Description The West Nile virus (WNV) is a mosquito-borne flavivirus causing meningoencephalitis in humans and animals. Due to their particular susceptibility to WNV infection, horses serve as a sentinel species. In a population of Romanian semi-feral horses living in the Danube delta region, we have analyzed the distribution of candidate polymorphic genetic markers between anti WNV-IgG seropositive and seronegative horses. Thirty-six SNPs located in 28 immunity-related genes and 26 microsatellites located in the MHC and LY49 complex genomic regions were genotyped in 57 seropositive and 32 seronegative horses. The most significant association (p(corr)<0.0002) was found for genotypes composed of markers of the SLC11A1 and TLR4 genes. Markers of five other candidate genes (ADAM17, CXCR3, IL12A, MAVS, TNFA), along with 5 MHC class I and LY49-linked microsatellites were also associated with the WNV antibody status in this model horse population. The OAS1 gene, previously associated with WNV-induced clinical disease, was not associated with the presence of anti-WNV antibodies.
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