Publication details

Cell-surface receptors enable perception of extracellular cytokinins

Authors

ANTONIADI Ioanna NOVÁK Ondřej GELOVÁ Zuzana JOHNSON Alexander PLÍHAL Ondřej SIMERSKÝ Radim MIK Václav VAIN Thomas MATEO-BONMATÍ Eduardo KARADY Michal PERNISOVÁ Markéta PLAČKOVÁ Lenka OPASSATHIAN Korawit HEJÁTKO Jan ROBERT Stéphanie FRIML Jiří DOLEŽAL Karel LJUNG Karin TURNBULL Colin

Year of publication 2020
Type Article in Periodical
Magazine / Source NATURE COMMUNICATIONS
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://www.nature.com/articles/s41467-020-17700-9
Doi http://dx.doi.org/10.1038/s41467-020-17700-9
Keywords cytokinin signalling
Description Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.
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