Publication details

Applicability of capillary electrophoresis-frontal analysis for displacement studies: effect of several drugs on L-tryptophan and lidocaine binding to human serum albumin

Authors

NEVÍDALOVÁ Hana MICHALCOVÁ Lenka GLATZ Zdeněk

Year of publication 2020
Type Article in Periodical
Magazine / Source Journa of Separation Science
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1002/jssc.202000594
Keywords CE, albumin, Drug
Description In this study, the binding characteristics of six drugs with human serum albumin, the most abundant protein in human plasma, were determined by capillary electrophoresis-frontal analysis, and the obtained values of binding parameters were compared with the literature data. The affect of several drugs and site markers on the binding of L-tryptophan and lidocaine to human serum albumin was investigated in subsequent displacement studies which thus demonstrated the usability of capillary electrophoresis as an automated high-throughput screening method for drug-protein binding studies.
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