Publication details

Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells

Authors

JASENSKÁ Daniela KAŠPÁRKOVÁ Věra RADASZKIEWICZ Katarzyna Anna CAPÁKOVÁ Zdenka PACHERNÍK Jiří TRCHOVÁ Miroslava MINAŘÍK Antonín VAJĎÁK Ján BÁRTA Tomáš STEJSKAL Jaroslav LEHOCKÝ Marián TRUONG Thanh Huong MOUČKA Robert HUMPOLÍČEK Petr

Year of publication 2021
Type Article in Periodical
Magazine / Source Carbohydrate Polymers
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.1016/j.carbpol.2020.117244
Doi http://dx.doi.org/10.1016/j.carbpol.2020.117244
Keywords Polyaniline; Polysacharides; Conducting composites; Human induced pluripotent stem cells
Description Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH). The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC). Moreover, the cardiomyogenesis and even formation of beating clusters were successfully induced on the films. The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues. The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 x 10(5) to < 1 cfu cm(-2). Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being similar to 1 and similar to 2 S cm(-1) for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH). The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.
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