Publication details
Parallel in vitro and in silico investigations into anti-inflammatory effects of non-prenylated stilbenoids
| Authors | |
|---|---|
| Year of publication | 2019 |
| Type | Article in Periodical |
| Magazine / Source | Food Chemistry |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1016/j.foodchem.2019.01.128 |
| Keywords | Anti-inflammatory; Cyclooxygenase; Molecular docking simulations; Lipoxygenase; Macrophages; NF-kappa B; Stilbenes |
| Description | Stilbenoids represent a large group of bioactive compounds, which occur in food and medicinal plants. Twenty-five stilbenoids were screened in vitro for their ability to inhibit COX-1, COX-2 and 5-LOX. Piceatannol and pinostilbene showed activity comparable to the zileuton and ibuprofen, respectively. The anti-inflammatory potential of stilbenoids was further evaluated using THP-1 human monocytic leukemia cell line. Tests of the cytotoxicity on the THP-1 and HCT116 cell lines showed very low toxic effects. The tested stilbenoids were evaluated for their ability to attenuate the LPS-stimulated activation of NF-kappa B/AP-1. Most of the tested substances reduced the activity of NF-kappa B/AP-1 and later attenuated the expression of TNF-alpha. The effects of selected stilbenoids were further investigated on inflammatory signaling pathways. Non-prenylated stilbenoids regulated attenuation of NF-kappa B/AP-1 activity upstream by inhibiting the phosphorylation of MAPKs. A docking study used to in silico analyze the tested compounds confirmed their interaction with NF-kappa B, COX-2 and 5-LOX. |