Publication details

Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris

Authors

KIZOVSKÝ Martin PILÁT Zdeněk MYLENKO Mykola HROUZEK Pavel KUTA Jan SKOUPY Radim KRZYŽÁNEK Vladislav HRUBANOVA Kamila ADAMCZYK Olga JEZEK Jan BERNATOVÁ Silvie KLEMENTOVA Tereza GJEVIK Alzbeta SILER Martin SAMEK Ota ZEMANEK Pavel

Year of publication 2021
Type Article in Periodical
Magazine / Source BIOSENSORS-BASEL
MU Faculty or unit

Faculty of Science

Citation
Web https://www.mdpi.com/2079-6374/11/4/115
Doi http://dx.doi.org/10.3390/bios11040115
Keywords selenium; algae; Raman spectroscopy; EDX; ICP-MS; bioaccumulation; Chlorella vulgaris
Attached files
Description Selenium (Se) is an element with many commercial applications as well as an essential micronutrient. Dietary Se has antioxidant properties and it is known to play a role in cancer prevention. However, the general population often suffers from Se deficiency. Green algae, such as Chlorella vulgaris, cultivated in Se-enriched environment may be used as a food supplement to provide adequate levels of Se. We used Raman microspectroscopy (RS) for fast, reliable, and non-destructive measurement of Se concentration in living algal cells. We employed inductively coupled plasma-mass spectrometry as a reference method to RS and we found a substantial correlation between the Raman signal intensity at 252 cm(-1) and total Se concentration in the studied cells. We used RS to assess the uptake of Se by living and inactivated algae and demonstrated the necessity of active cellular transport for Se accumulation. Additionally, we observed the intracellular Se being transformed into an insoluble elemental form, which we further supported by the energy-dispersive X-ray spectroscopy imaging.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info