Publication details
Preparative protein concentration from biofluids by epitachophoresis
| Authors | |
|---|---|
| Year of publication | 2022 |
| Type | Peer-reviewed scientific article |
| Magazine / Source | Journal of Chromatography A |
| MU Faculty or unit | |
| Citation | |
| web | https://www.sciencedirect.com/science/article/pii/S0021967322007828?via%3Dihub |
| Doi | https://doi.org/10.1016/j.chroma.2022.463591 |
| Keywords | Proteins; Epitachophoresis; Concentration; Urine; Growth medium |
| Description | With increasing demands on protein analyses in complex biological matrices, the insistence on developing new sample preparation techniques is rising. Recently, we introduced a new displacement electrophoresis technique (epitachophoresis) and instrumentation for preparative concentration and cleaning of DNA samples. This work describes the possibility of applying this device to protein samples. We have developed a method for the epitachophoretic concentration of proteins in a cationic mode and tested it by concentrating and collecting the protein zones from complex biological matrices (urine and growth medium). Under optimized conditions, we have obtained recoveries up to 99%. Furthermore, the applicability of the developed method was proven by concentrating and collecting the cytochrome c zone from a HeLa cell line growth medium, where the protein cytochrome c was released during cell apoptosis. |