Publication details
Translation initiation stages of coupled transcription-translation
| Authors | |
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| Year of publication | 2022 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Transcription and translation are functionally coupled and highly coordinated in most bacterial species, with few exceptions such as Bacillus subtilis. Translational riboswitches (cis-acting RNA structures) located in the 5-untranslated region of mRNAs can modulate transcription pausing or premature termination. They can regulate both transcription and translation in a ligand-dependent manner and serve as an excellent model system to investigate the coordination of coupled transcription-translation. Here we use a pre-queuosine1 (PreQ1) sensing riboswitch to understand the coordination of translation initiation and RNA polymerase (RNAP) pausing during transcription. Using cryo-electron microscopy as a primary approach we investigate the mRNA loading mechanism onto small ribosomal subunit (30S) and the coordination of translation initiation and transcription elongation. Our specific focus is to understand the dynamics of 30S binding to the mRNA riboswitch containing ribosomal binding site, formation and stabilization of pre-initiation complex and subsequent transient interactions during the initial steps of transcription-translation coupling. |
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