Publication details

Telomerase RNA in Hymenoptera (Insecta) switched to plant/ciliate-like biogenesis

Authors

FAJKUS Petr ADÁMIK Matej NELSON Andrew D L KILAR Agata Magdalena FRANEK Michal BUBENÍK Michal FRYDRYCHOVA Radmila Capkova VOTAVOVA Alena SYKOROVA Eva FAJKUS Jiří PESKA Vratislav

Year of publication 2023
Type Article in Periodical
Magazine / Source Nucleic acids research
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://academic.oup.com/nar/article/51/1/420/6956357
Doi http://dx.doi.org/10.1093/nar/gkac1202
Keywords TEMPLATE-BOUNDARY DEFINITION;PSEUDOKNOT;SEQUENCE;IDENTIFICATION
Description In contrast to the catalytic subunit of telomerase, its RNA subunit (TR) is highly divergent in size, sequence and biogenesis pathways across eukaryotes. Current views on TR evolution assume a common origin of TRs transcribed with RNA polymerase II in Opisthokonta (the supergroup including Animalia and Fungi) and Trypanosomida on one hand, and TRs transcribed with RNA polymerase III under the control of type 3 promoter, found in TSAR and Archaeplastida supergroups (including e.g. ciliates and Viridiplantae taxa, respectively). Here, we focus on unknown TRs in one of the largest Animalia order - Hymenoptera (Arthropoda) with more than 300 available representative genomes. Using a combination of bioinformatic and experimental approaches, we identify their TRs. In contrast to the presumed type of TRs (H/ACA box snoRNAs transcribed with RNA Polymerase II) corresponding to their phylogenetic position, we find here short TRs of the snRNA type, likely transcribed with RNA polymerase III under the control of the type 3 promoter. The newly described insect TRs thus question the hitherto assumed monophyletic origin of TRs across Animalia and point to an evolutionary switch in TR type and biogenesis that was associated with the divergence of Arthropods.
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