Publication details

In cellulo and in vivo assays for compound testing against Trypanosoma cruzi


GUERRA-SLOMPO Eloise Pavao PICCHI-CONSTANTE Gisele Fernanda Assine MAREK Martin ROMIER Christophe SIPPL Wolfgang ZANCHIN Nilson Ivo Tonin

Year of publication 2023
Type Article in Periodical
Magazine / Source STAR Protocols
MU Faculty or unit

Faculty of Science

Keywords Cell Biology; Cell-based Assays; High-throughput Screening; Microbiology; Model Organisms
Description Here, we describe a combined in cellulo and in vivo approach to identify com-pounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determina-tion. Compounds showing high SI are tested using in vivo infection models in par-allel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment. For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).1

You are running an old browser version. We recommend updating your browser to its latest version.

More info