Publication details
In cellulo and in vivo assays for compound testing against Trypanosoma cruzi
| Authors | |
|---|---|
| Year of publication | 2023 |
| Type | Article in Periodical |
| Magazine / Source | STAR Protocols |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.sciencedirect.com/science/article/pii/S2666166723000163?via%3Dihub |
| Doi | http://dx.doi.org/10.1016/j.xpro.2023.102058 |
| Keywords | Cell Biology; Cell-based Assays; High-throughput Screening; Microbiology; Model Organisms |
| Description | Here, we describe a combined in cellulo and in vivo approach to identify com-pounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determina-tion. Compounds showing high SI are tested using in vivo infection models in par-allel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment. For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).1 |