Publication details
Improvement of concentration sensitivity in capillary electrophoresis-frontal analysis
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| Year of publication | 2023 |
| Type | Conference abstract |
| Citation | |
| Description | Capillary electrophoresis is a powerful technique with many advantages. However, it suffers from the lack of concentration sensitivity associated with common utilisation of UV-VIS detector. The issue can be solved by replacing the detector with a more sensitive one or incorporation a preconcentration step without changing the detection instrument. Established capillary electrophoresis method for binding studies which gives information not only about binding strength but also about number of binding sites is capillary electrophoresis-frontal analysis (CE-FA). This study is focused on interaction between plasma protein and drug as a small molecule. For improvement of concentration sensitivity in CE-FA method the contactless conductivity detector is chosen and the second strategy enhancement of the UV-VIS detection by on-line preconcentration step field amplified sample stacking which amplifies concentration sensitivity in capillary during separation is used. For each newly developed method the conventional CE-FA UV-VIS method is optimised and binding parameters are compared. |
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