Publication details
Detekce bakterií Leptospira interrogans s.l. u malých savců
| Title in English | Detection of Leptospira interrogans s.l. bacteria in small mammals |
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| Authors | |
| Year of publication | 2024 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | The presentation deals with the group of bacteria Leptospira interrogans sensu lato. This is a pathogenic group of leptospires that cause the zoonotic disease leptospirosis, which in some of its forms can be fatal to humans. The most common hosts of these bacteria are small mammals, mainly rodents. The method discussed in the presentation is MAT (microscopic agglutination test). This is an indirect method of detecting leptospires that detects the presence of antibodies against the bacteria. This method is considered the gold standard for detecting leptospires. It is based on the reaction of all classes of antibodies from the body of the examined mammals with live strains of leptospires. After cultivation, the bacteria are observed under a microscope in a dark field, when it is determined whether agglutination has occurred. Samples obtained from small mammals were obtained from captures in the Moravian Karst (permitted by the Ministry of the Environment project). Samples from these animals were used to detect antibodies against which 9 different leptospire serovars occurring in the Czech Republic were used. So far, 75 samples have been examined. 18 of them were found to be positive at a titer of 1:100, which is 24% positivity. The most common serovars were sejroe and grippotyphosa. These serovars are described as the most frequently occurring in rodents. From these and other results, it will be possible to deduce the prevalence of Leptospira interrogans s.l. in the Moravian Karst Protected Area, the individual most frequently occurring serovars in this area, and the risk of transmission to humans. |
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