Publication details
New fluorescent markers for glycan analysis
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Changes in the composition of glycans correlate with the progression of many diseases, therefore they are studied as disease markers. However, their analysis is difficult due to the absence of chromophores. Our goal was to develop a series of new fluorescent markers covalently bound to glycans providing fast labeling kinetics and high quantum yield, which will allow to study glycans chromatographically (HPLC). The skeleton of these fluorophores is based on pyrroindolizine. There are two building blocks, synthon A and synthon B, which were subsequently connected and bridged by a carbonyl fragment. The plan was to prepare variants of the fluorophore carrying electron-donating (Me, MeO) or electron-withdrawing (NO2, F) groups on synthon A. These substitutions allow for variability in the wavelength of the absorption maximum and thus the wavelength of fluorescence. The side chain essential for attachment of the fluorophore to the glycan was modified to a hydrazide (neutral glycans labeling) or an activated ester with N-hydroxysuccinimide (aminoglycans labeling). The prepared structures will be characterized in cooperation with the Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic. The usability of the newly developed fluorophores will be demonstrated by profiling glycoproteins associated with breast cancer. |
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